Purify, desalt, and concentrate with a single tip
2N1 IMCStips® integrates affinity purification and desalting within a single tip, removing the need for separate consumables and transfer steps. This format enables higher recovery and efficient concentration of dilute samples in a single run.
Why choose 2N1 IMCStips®?
- Two types of purification with one tip
- Up to 96 samples processed in 90 minutes or less
- Higher recoveries in less time
- Concentration of dilute samples
As with every IMCStips product, 2N1 is backed by direct, responsive support from the scientists who built it.
Eliminate multiple consumables with 2N1
Figure 1. The 2N1 IMCStips workflow.
2N1 follows the familiar IMCStips bind-wash-elute workflow. After equilibration, the sample binds to the top resin layer (affinity or ion exchange) while contaminants and salts make their way through the SizeX (buffer exchange) layer. Multiple washes serve to equilibrate the SizeX resin while removing any non-bound cellular material. Standard elution buffers are used to elute the sample into the SizeX layer where it is exchanged into the desired buffer formulation.
Automated purification, desalting, and concentration with 2N1 IMCStips®
Figure 2. Automated affinity purification and buffer exchange using 2N1 IMCStips vs. sequential purification (Ni-IMAC + SizeX150 IMCStips) on a Hamilton STAR. Starting material ranged from 200-800 µg, with n=8 for the Ni-IMAC + SizeX150 workflow and n=6 for 2N1 IMCStips. Student t-tests indicated no significant differences between the two workflows (p>0.05) except at the 800 µg starting amount (p=0.04).
Internal data comparing the 2N1 IMCStips (affinity + buffer exchange resins) workflow with a sequential method using Ni-IMAC Affinity IMCStips followed by SizeX150 IMCStips show that 2N1 produces recoveries comparable to or better than the two-step approach on a Hamilton STAR liquid handler (Figure 2). Another study evaluating 2N1 IMCStips (ion exchange + buffer exchange resins) for His₆-tagged GFP purification demonstrate recoveries consistently above 80% on a Tecan Fluent, with the full concentration and buffer exchange workflow completed in under 30 minutes (Figure 3).
Figure 3. Recoveries of His₆-tagged GFP using 2N1 IMCStips containing SizeX150 buffer exchange resin and cation exchange resin on the Tecan Fluent. Recoveries were 91.7 ± 3.6%, 85.5 ± 3.3%, 88.8 ± 1.8%, and 83.8 ± 2.0% (n=4) for starting material ranging from 190-1330 µg. The complete concentration and buffer exchange workflow was completed in under 30 minutes.
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Figure 4. Purification of His6-GFP from cell lysates using 2N1 IMCStips. Initial protein loaded ranged from 200 to 1200 μg (1.5 mg/mL). (A) Eluate volumes, (B) protein recoveries, and (C) eluate concentrations are presented as means ± SD. Individual data points are shown relative to the group mean and one standard deviation.
2N1 IMCStips (Ni-IMAC + SizeX) used to purify His6-tagged GFP on a Hamilton STAR show consistent eluate volumes, recovery percentages, and protein concentrations (Figure 4).
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