OVERVIEW
IMCStips for Automated Phosphopeptide Enrichment are designed for automated high throughput with a customizable screening workflow. We offer automated scripts that allow the user to screen different wash and elution buffers and a number of different resins in a single experiment.
Using metal oxides, phosphopeptides from cell lysates are enriched. The global enrichment of these phosphoserine and phosphothreonine peptides is optimized using zirconium dioxide and PolyTi™ resins in IMCStips.
BENEFITS
- Specifically designed to enhance global enrichment of phosphorylated proteins and peptides, ensuring a comprehensive analysis
- Capable of efficiently enriching and purifying phosphopeptides from samples in as little as 30 minutes, significantly enhancing lab throughput
- Ensures high specificity in the isolation of targets, significantly reducing non-specific binding thanks to the pre-optimized protocol
- Comes with templated or customized scripts that are ready to integrate into existing workflows, eliminating the need for time-consuming method development
Our pipette tips support Phosphopeptide applications using these resins:
- PolyTi™
View and download our product sheet below:
The different distribution of singly-, doubly- and triply- phosphorylated peptides enriched using the different resins.
Venn diagrams of phosphopeptide overlap from duplicate sample enrichments. The duplicate sample preparations for PolyTi (A), ZrO2 (B) and the combination of both resins in a single pipette tip (C) show an average of 76, 78, and 71 percent overlap, respectively. In comparison, the two different resins PolyTi and ZrO2 show 48 percent of overlap (D). This reduction in overlap of phosphopeptide coverage is expected for these two resin types, as the distribution of the singly- and doubly-phosphorylated peptides were also varied for the two resin types.
