IMCStips Automated Size Exclusion | Automated Buffer Exchange

OVERVIEW

SizeX IMCStips® are miniaturized size exclusion chromatography columns designed for fully automated, rapid buffer exchange. SizeX IMCStips are packed with hydrophilic polymeric resin, allowing for remarkable chemical and physical stability for various size exclusion chromatography applications.

AUTOMATION PLATFORMS

SizeX IMCStips are compatible with various automated liquid handling platforms, including Hamilton, Dynamic Devices, Tecan, Opentrons, and Analytik Jena. Our custom scripting solutions tailor the method to your specific needs, ensuring seamless integration into your laboratory workflow. With on-site or remote installation and unparalleled technical support, we will work closely with you to make this process as smooth as possible. SizeX IMCStips enables you to process samples in less than 10 minutes, saving you considerable time, money, and effort.

Figure 1. Fully Automated Size Exclusion Workflow with SizeX IMCStips

BENEFITS

Eliminate centrifugation requirements for buffer exchange
Precise flow rate controls to achieve ideal size separations
Fully automated process with little or no manual intervention
Readily coupled with additional automated workflows such as multi-attribute method, recombinant protein purification, and IgG purification.

Unlike manual spin columns, automated size exclusion chromatography with SizeX IMCStips enable massive time savings, excellent reproducibility and reliability without sacrificing sample recoveries.^1,2,3

Parts of a SizeX IMCStip for automated size exclusion workflow

Kates, P. A., Cook, J. N., Ghan, R., Nguyen, H. J., Sitasuwan, P. & Lee, L. A. (2023) Incorporation of automated buffer exchange empowers high-throughput protein and plasmid purification for downstream uses. SLAS Technology. 28: 243–250.

Resin Options Catalog Specifications Poster Presentations Webinars Journal Articles

Resin Options

SizeX₁₀₀ - Hamilton, Dynamic Devices, Tecan
SizeX₁₅₀ - Hamilton, Dynamic Devices, Tecan
SizeX₇₅₀ - Dynamic Devices
SizeX₁₀₀₀ - Dynamic Devices

View and download our product sheets below:

Catalog

Specifications

Product Compatibility

SizeX IMCStips can process samples in both solubilizing and denaturing conditions. The ionic strength of the sample and eluant should be at least 20 mM to eliminate the effect of negatively charged groups on the sorbent. For protein separation, buffers with > 50 mM ionic strength is recommended. Compatibility chart below includes chemical examples but not limited to the list. Avoid strong oxidizing agents when using this product.

Compatibility	Examples
pH	2-10
Temperature	4-80°C
Alcohol	Up to 20 percent
Chaotropic agents	Up to 6-M guanidine
Reducing agents	Dithiothreitol, Mercaptoethanol
Detergents	Sodium dodecyl sulfate (SDS), CHAPS, Triton X-100
Volatile buffer salts	Pyridine, acetic acid, ammonium formate, ammonium bicarbonate

Poster Presentations

Webinars

Journal Articles

Kates PA, Cook JN, Ghan R, Nguyen HJ, Sitasuwan P, Lee LA. (2023). Incorporation of automated buffer exchange empowers high-throughput protein and plasmid purification for downstream uses. SLAS technology. doi: 10.1016/j.slast.2023.01.005.
Ogata Y, Quizon PM, Nightlinger NS, Sitasuwan P, Snodgrass C, Lee LA, Meyer JD, Rogers RS. (2021). Automated multi-attribute method sample preparation using high-throughput buffer exchange tips. Rapid communications in mass spectrometry. doi: 10.1002/rcm.9222.
Sitasuwan P, Powers TW, Medwid T, Huang Y, Bare B, Lee LA. (2021). Enhancing the multi-attribute method through an automated and high-throughput sample preparation. mAbs. 13(1):1978131. doi: 10.1080/19420862.2021.1978131.

HIGH PERFORMANCE AND CONSISTENCY

(A) Average percentages of recovered antibody concentration, volume, and total antibody amount (n=20). Error bar shows ± 1 S.D. (B) After the digestion, the sample was analyzed on HPLC-UV. Eight representative A280 chromatograms are overlaid. (C) Percent error of area integration of 8 replicates.

MAM Workflow Using SizeX IMCStips®

Our tips are designed for automated buffer exchange and to work with chaotropic salts such as 6-M guanidine. This process, typically implemented in bioanalytical procedures such as multi-attribute method (MAM), allows for the efficient reduction of guanidine prior to trypsin/LysC digestion. The resins contained in these IMCStips® lack absorptive properties which provide high recoveries of biomolecules.

Automated MAM Workflow using IMCStips for automated size exclusion

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SIZE EXCLUSION