Sulfazyme DS - IMCS | Purified Sulfatase for DHEAS Hydrolysis

Sulfazyme DS (EC 3.1.6.1) is a purified recombinant arylsulfatase variant to Sulfazyme PaS. Sulfazyme DS is specifically designed to hydrolyze dehydroepiandrosterone sulfate (DHEAS).

Choose between Sulfazyme™ PaS for general sulfatase needs, genetically engineered Sulfazyme DS for hydrolyzing dehydroepiandrosterone sulfate (DHEAS), or genetically engineered Sulfazyme β-AS for hydrolyzing boldenone sulfate, and testosterone sulfate. Additionally, Sulfazyme™ can be paired with our beta-glucuronidase to analyze both glucuronidated and sulfated metabolites simultaneously.

Product Specifications How to Use Catalog Posters Journal Articles App Notes

Product Specifications

PRODUCT SPECIFICATIONS: [DOWNLOAD]

Physical Description:	Clear aqueous solution
Specific Activity:	≥3.5 U/mg*
Storage Temperature:	2-8 °C

*One unit is equivalent to 1.0 micromole of 4-nitrocatechol sulfate hydrolyzed per minute at pH 8.0 and 25°C.

How to Use

Recommended reaction set up for DHEA sulfate hydrolysis

Add MilliQ water to urine samples.
Add 1 M Tris-HCl, pH 8.0 Buffer.
Add internal standard or additional MilliQ water.
Add Sulfazyme™ DS and mix gently.
DO NOT VORTEX.
Incubate at 37°C for 4 hours

Small Volume Hydrolysis Reaction Conditions	No enzyme (unhydrolyzed urine blank) (µL)	Sulfazyme™ (Pas, DS, or β-AS) only (μL)	B. pi β-GUS only (µL)	Sulfazyme™ (Pas, DS, or β-AS) and B. pi β-GUS (μL)
Urine	200	200	200	200
Suifazyme™ Buffer (1.0 M Tri s-HCl pH 8)	30	30	30	30
Internal Standard (ISTD, or use MilliQ water)	20	20	20	20
MilliQWater	180	160	160	140
Sulfazyme™ (Pas, DS, or β-AS)	0	20	0	20
β-glucuronidase (B. pi β-glucuronidase variant)	0	0	20	20
Total Volume (µL)	430	430	430	430