Sulfazyme β-AS - IMCS | Purified Sulfatase for Androgen Steroid Hydrolysis

Sulfazyme™ β-AS (EC 3.1.6.1) is a purified recombinant variant of arylsulfatase derived from Sulfazyme™ PaS, specifically designed to hydrolyze boldenone sulfate and testosterone sulfate.

Choose between Sulfazyme™ PaS for general sulfatase needs, genetically engineered Sulfazyme DS for hydrolyzing dehydroepiandrosterone sulfate (DHEAS), or genetically engineered Sulfazyme β-AS for hydrolyzing boldenone sulfate, and testosterone sulfate. Additionally, Sulfazyme™ can be paired with our beta-glucuronidase to analyze both glucuronidated and sulfated metabolites simultaneously.

Product Specifications How to Use Catalog Posters Journal Articles App Notes

Product Specifications

PRODUCT SPECIFICATIONS: [DOWNLOAD]

Physical Description:	Clear aqueous solution
Specific Activity:	≥6.0 U/mg*
Storage Temperature:	2-8 °C

*One unit is equivalent to 1.0 micromole of 4-nitrocatechol sulfate hydrolyzed per minute at pH 8.0 and 25°C.

How to Use

Recommended reaction set up for boldenone sulfate and testosterone sulfate hydrolysis:

Add MilliQ water to urine samples.
Add 1 M Tris-HCl, pH 8.0 Buffer.
Add internal standard or additional MilliQ water.
Add Sulfazyme™ β-AS and mix gently.
DO NOT VORTEX.
Incubate at 37°C for 4 hours

Hydrolysis Reaction Conditions	No enzyme (Unhydrolyzed urine blank) (µL)	Sulfazyme™ only (PaS, DS or β-AS) (μL)	B. pi β-GUS only (µL)	Sulfazyme™ (PaS, DS or β-AS) and B. pi β-GUS (μL)
Urine	200	200	200	200
Buffer (1.0 M Tris-HCl, pH 8.0)	30	30	30	30
Internal standard (or use MilliQ water)	20	20	20	20
MilliQ water	180	160	160	140
Sulfazyme™ (Pas, DS or β-AS)	0	20	0	20
Glucuronidase (B. pi β-glucuronidase variant)	0	0	20	20
Total Volume (µL)	430	430	430	430

Note: Some steroids are sensitive to diluent and mobile phase conditions. Estradiol exhibits ion suppression in the presence of formic acid.

Catalog

Posters

Journal Articles

Helmer E, Karimian N, Van Assche K, Seghers I, Le Tallec S, Cherala G, Scott G, Namour FS. (2022). Ziritaxestat Drug–Drug Interaction with Oral Contraceptives: Role of SULT1E1 Inhibition. Clinical Pharmacology & Therapeutics. doi: 10.1002/cpt.2689
Lessard-Lord J, Plante PL, Desjardins Y. (2022). Purified recombinant enzymes efficiently hydrolyze conjugated urinary (poly)phenol metabolites. Food & Function. doi: 10.1039/d2fo02229j
Wang FR, Fei J, Yu XL, Zhao XC, Wang Q, Metavarayuth K. (2018). Advancing the Analysis of Terbutaline in Urine Samples Using Novel Enzyme Hydrolysis. Bioanalysis. doi: 10.4155/bio-2018-0145
- A summary of "Advancing the analysis of terbutaline in urine samples using novel enzyme hydrolysis"

App Notes

Product Description	Catalog Number
Sulfazyme™ β-AS - 5 mL	04E-200-005

PRODUCT SPECIFICATIONS: [DOWNLOAD]

Order Sulfazyme™ β-AS here!