Simplifying Urine Drug Testing with “Flash Hydrolysis”
5 µL vs. 100 µL. ONLY ONE FINISHED THE JOB.
IMCSzyme® RT achieved >90% hydrolysis in 15 minutes using 20x less enzyme.
- IMCSzyme® RT hydrolyzed >90% of morphine, oxymorphone, and codeine glucuronides within 15 minutes at room temperature
- Achieved results using just 5 μL, which is 1/20th the enzyme volume of a competing product
- The competing enzyme left significant unhydrolyzed substrate in two of three analytes tested, even at 100 μL (2:1 enzyme-to-urine ratio)
STUDY OVERVIEW
Dr. Tania Sasaki at Altius Diagnostics evaluated hydrolysis efficiency under demanding conditions: 20,000 ng/mL each of morphine, oxymorphone, and codeine glucuronides in 50 µL of urine (60,000 ng/mL of glucuronidated substrate total).
Each enzyme manufacturer's recommendations were used for the hydrolysis conditions: 5 µL for IMCSzyme RT and 100 µL for the competing "all-in-one" premixed enzyme. Remaining glucuronides were monitored at 8-minute intervals at room temperature.
RESULTS
IMCSzyme RT hydrolyzed greater than 90% of all three glucuronides within 15 minutes.
The "all-in-one" premixed enzyme, at 20 times the volume, still had 23% of oxymorphone glucuronide and 60% of codeine glucuronide remaining.
ABOUT THE STUDY
This poster was presented by Dr. Tania Sasaki at the October 2019 Annual Meeting of the Society of Forensic Toxicologists in San Antonio, TX. Click the button below to download the full poster.
This poster is a head-to-head comparison of IMCSzyme RT vs. a commercially available "all-in-one" β-glucuronidase.