Multi-attribute method (MAM) is a mass spectrometry-based technique that enables users to simultaneously monitor and quantify molecular attributes with the potential to impact the efficacy, pharmacokinetics, and safety of biotherapeutic applications. From proof-of-concept and product development to real-time quality control, MAM is intended to consolidate separate assays and streamline laboratory workflows.
Current sample preparation for MAM relies on a manual buffer exchange step to remove excess denaturant and accelerate subsequent trypsin digestion efficiency. Circumventing the buffer exchange step requires significant sample dilution and prolonged digestion time, which may result in sample preparation artifacts. Through the use of SizeX IMCStips on Hamilton Microlab STAR platforms, automated buffer exchange for MAM is an easy and hands-free, walkaway method.
Learn how SizeX IMCStips and Hamilton Microlab STAR platforms could help streamline your automated MAM sample preparation workflow.
Click the link below to access the full brochure and make sure to follow us on LinkedIn!
Enhancing the Multi-Attribute Method through an Automated and High-throughput Sample Preparation
Our recent peer-reviewed research published in the journal mAbs describes a completely automated multi-attribute method (MAM) sample preparation approach. For the first time, a complete tip-based MAM sample preparation protocol is automated on a single robotic liquid handling system, leveraging a deck layout that integrates both heating and cooling functionalities. Rapid desalting with SizeX IMCStips® is achieved with Hamilton Robotics. The fully automated method achieves high-throughput sample preparation for MAM, while maintaining superior method performance.
Automated Multi-Attribute Method Sample Preparation using High-Throughput Buffer Exchange Tips
Our latest peer-reviewed research published in the journal Rapid Communications in Mass Spectrometry describes a fully automated multi-attribute method (MAM) sample preparation protocol. Rapid desalting in less than 15 minutes is achieved using miniaturized size exclusion chromatography columns (SizeX IMCStips®) on an automated liquid handler (a Hamilton Microlab STAR). This automated protocol enables the preparation of up to 96 samples with less “at-bench” time than the manual preparation of a smaller batch of samples, thereby greatly facilitating support of process development and the use of MAM in QC.
IMCS Scientists Help Local Community Fight COVID-19
