IMCSzyme® Product Line - IMCS | The Science of Reliability

IMCSzyme® is a genetically modified beta-glucuronidase used to detect a wide range of drugs by hydrolyzing glucuronides in biological fluids. After rapid hydrolysis with IMCSzyme, samples can be analyzed by immunoassay, mass spectrometry, and high-performance liquid chromatography. IMCSzyme hydrolyzes multiple drug classes with higher efficiency as compared to other available enzymes.

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CONSISTENT, STABLE, AND PURE ENZYMES

Made in the USA and used to hydrolyze more than one million samples each month. IMCSzyme is the first genetically modified pure beta-glucuronidase. Our pure enzyme hydrolyzes multiple drug classes quickly and with high efficiency. We have an extensive quality control program and manufacturing process that ensures consistency across lots. IMCSzyme is shipped overnight and stored at 2 - 8°C.

CLINICAL TOXICOLOGY: Our enzyme has proven to increase the percentage of drugs hydrolyzed, therefore increasing the accuracy of test results. In addition, IMCSzyme decreases incubation time, enabling same-day test results.

PAIN CARE: Fast comprehensive hydrolysis is critical to maintaining an efficient workflow in testing for pain care drugs.

FORENSIC TOXICOLOGY: Crime labs have discovered using IMCSzyme for hydrolysis in blood, urine, or tissue increases the detection of codeine and other hard to hydrolyze analytes. Our pure enzyme does not contain contaminants that lead to the conversion of 6-monoacetylmorphine (6-MAM) to morphine, among other conversions.

IMCSzyme has been featured in poster presentations at AAC, MSACL, SOFT, AAFS, ASMS, and published in the Journal of Analytical Toxicology.

Clean, fast, and comprehensive hydrolysis in less than 30 minutes
Reduce matrix effects - fewer interferents
Decrease the preparation times and increase throughput

View our additional IMCSzyme studies and resources here.

IMCSzyme® Product Specifications

E1F 3S 3P

E1F

IMCSzyme® E1F [DOWNLOAD]

Physical Description:	Clear aqueous solution
*Specific Activity (units/mL)**:	>50,000
Guaranteed Shelf Life:	12 months
Storage Temperature:	2-8°C

*One modified Fishman unit will liberate 1 microgram of phenolphthalein from phenolphthalein glucuronide per hour at 25°C at pH 6.8

Image of a graph of data from patient urine containing excess glucuronides. The analytes included in this table are THCS, Amitriptyline, Benzodiazepine, Norbuprenorphine, Morphine-3, Temazepam, and Codeine-6. — **TESTED ON REAL PATIENT URINE CONTAINING EXCESS GLUCURONIDES TO PRODUCE ACCURATE RESULTS.**

Graph showing no incubation with beta-glucuronidases

Graph shows 30-minute Incubation with beta-glucuronidases

RAPID HYDROLYSIS BUFFER INCLUDED IN EVERY ORDER

The use of our complimentary Rapid Hydrolysis Buffer ensures proper pH for optimal hydrolysis conditions with IMCSzyme.

Frequently Asked Questions

Need help? Check out our FAQ section below or contact us for immediate assistance!

Does IMCSzyme® convert 6-MAM to morphine?

How effective is IMCSzyme® towards glucuronidated benzodiazepines?

What if the urine sample has a pH above 9?

Should I adjust the temperature or incubation time if I incubate my sample in a water bath or a heating block as compared to a convection oven?

Yes, incubating in a water bath or a heating block will benefit from a lower temperature due to differences in heat transfer efficiencies.

For IMCSzyme®:

When using a water bath or heating block, incubate at 45 °C for 30 minutes.
When using a convection oven, heat transfer is slower, hence setting the temperature from 55 to 60 °C and incubate for 30 minutes.

IMCSzyme® RT does not require heating and is able to effectively hydrolyze samples at room temperature. Heating of IMCSzyme® RT does not increase hydrolysis efficiency.

Can I dilute my enzyme and just let the hydrolysis reaction run longer?

We do not recommend diluting the enzyme and incubating longer. At a lower enzyme concentration, the stability of the enzyme in the reaction will become more of a factor. Urine samples are known to contain inhibitors that can inactivate the efficiency of any enzyme over time. If the enzyme concentration is too low, there is a strong likelihood that the reaction would not go to completion during the longer incubation, as the enzyme would be inactivated before the time expires. This could result in a false negative result. At the suggested enzyme concentration and shorter incubation time, the enzyme can withstand a hostile sample long enough to complete hydrolysis.