The Multi-Attribute Method (MAM) is an application of ultrahigh-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) for simultaneous detection, identification, quantitation, and monitoring of molecular attributes of biotherapeutics. Critical Quality Attributes (CQAs) are identified based on the severity of harm to patient, which include efficacy (potency), pharmacokinetics, and safety/immunogenicity. MAM is a single MS-based method used to monitor known CQAs as well as to detect new peaks, which are indicative of impurities. This approach has allowed the introduction of MS into the quality control (QC) environment of biotherapeutics manufacturing.
Casey Snodgrass, PhD, pharmaceutical biotechnology market segment leader for the Hamilton Company expands on this in a recent interview with GEN. Dr. Snodgrass talks about collaborating with IMCS and using SizeX IMCStips to develop automated sample preparation workflows using the Hamilton Automated Liquid Handling platform. SizeX IMCStips are specialized size exclusion chromatography pipette tips that are built for automation platforms like the Hamilton STAR liquid handling system, leading to hands-free sample preparation, optimized workflows, and faster turnaround times.
Nikki Sitasuwan, PhD, senior research scientist at IMCS, presented a poster at CASS Symposium – Mass Spec 2019 on automating MAM sample preparation using SizeX IMCStips on a Hamilton Microlab STAR. Automated MAM sample prep using SizeX IMCStips paired with the Hamilton STAR generated highly consistent and robust results comparable to tedious manual preparation. This workflow was validated by two independent laboratories.
For more information, download the automated MAM sample preparation poster here.
Curious about SizeX IMCStips? Click here to learn more.
Dr. Andrew Lee discusses challenges and solutions in antibody purification with The Scientists’ Channel
