STREAMLINE YOUR PURIFICATIONS WITH SUPERIOR REPRODUCIBILITY AND REDUCED HANDS-ON TIME
IMCStips is a patented dispersive solid-phase extraction technology used in the field of proteomics to provide simplified, highly efficient sample preparations. IMCStips contain loose resin that increase the efficiency of your workflows by facilitating the enhanced binding of analytes of interest during pipetting steps. By coupling this technology with the INTEGRA VIAFLO 96, ASSIST and ASSIST PLUS electronic pipetting platforms, workflows are streamlined with the hands-free implementation of multiple aspirate and dispense cycles that facilitate consistent results, high recoveries, and faster workflows.
BENEFITS
- High reproducibility with error-free pipetting
- Reduced hands-on time and unmatched ergonomics
- Dispersive solid phase extraction (dSPE) pipette tips facilitate maximum contact between resin and analyte leading to higher recoveries in less time
| AFFINITY | CUSTOM |
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| Resin Options | Resin Options |
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| Applications | Applications |
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| VIEW | VIEW |
| Tip Size | Resin Amount | Tip Quantity | Catalog Number | Category | Platform | Resin | Resin Type | Sample Volume |
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| 1250 µL | 50 µL | 8 | 04T-I1R88F-1-50-8 | Affinity | Integra | Strep-Tactin®XT 4Flow® high capacity | Strep-Tactin | |
| 1250 µL | 50 µL | 96 | 04T-I1R88F-1-50-96 | Affinity | Integra | Strep-Tactin®XT 4Flow® high capacity | Strep-Tactin | |
| 1250 µL | 50 µL | 8 | 04T-I1R89-1-50-8 | Affinity | Integra | M2 Affinity Gel | M2 Affinity | |
| 1250 µL | 50 µL | 96 | 04T-I1R89-1-50-96 | Affinity | Integra | M2 Affinity Gel | M2 Affinity | |
| 1250 µL | 50 µL | 8 | 04T-I1R90F-1-50-8 | Affinity | Integra | Glutathione Sepharose 4 Fast Flow GST-tagged | Glutathione | |
| 1250 µL | 50 µL | 96 | 04T-I1R90F-1-50-96 | Affinity | Integra | Glutathione Sepharose 4 Fast Flow GST-tagged | Glutathione | |
| 1250 µL | 25 µL | 8 | 04T-I1R80-1-25-8 | Affinity | Integra | MabSelect SuRe LX | Protein A | |
| 1250 µL | 25 µL | 96 | 04T-I1R80-1-25-96 | Affinity | Integra | MabSelect SuRe LX | Protein A | |
| 1250 µL | 50 µL | 8 | 04T-I1R80-1-50-8 | Affinity | Integra | MabSelect SuRe LX | Protein A | |
| 1250 µL | 50 µL | 96 | 04T-I1R80-1-50-96 | Affinity | Integra | MabSelect SuRe LX | Protein A | |
| 1250 µL | 25 µL | 8 | 04T-I1R80A-1-25-8 | Affinity | Integra | MabSelect PrismA | Protein A | |
| 1250 µL | 25 µL | 96 | 04T-I1R80A-1-25-96 | Affinity | Integra | MabSelect PrismA | Protein A | |
| 1250 µL | 50 µL | 8 | 04T-I1R80A-1-50-8 | Affinity | Integra | MabSelect PrismA | Protein A | |
| 1250 µL | 50 µL | 96 | 04T-I1R80A-1-50-96 | Affinity | Integra | MabSelect PrismA | Protein A | |
| 1250 µL | 25 µL | 8 | 04T-I1R72XL-1-25-8 | Affinity | Integra | Ni Sepharose excel | Ni-IMAC | |
| 1250 µL | 25 µL | 96 | 04T-I1R72XL-1-25-96 | Affinity | Integra | Ni Sepharose excel | Ni-IMAC | |
| 1250 µL | 50 µL | 8 | 04T-I1R72XL-1-50-8 | Affinity | Integra | Ni Sepharose excel | Ni-IMAC | |
| 1250 µL | 50 µL | 96 | 04T-I1R72XL-1-50-96 | Affinity | Integra | Ni Sepharose excel | Ni-IMAC | |
| 1250 µL | 25 µL | 8 | 04T-I1R72-1-25-8 | Affinity | Integra | Ni Sepharose 6 Fast Flow | Ni-IMAC | |
| 1250 µL | 25 µL | 96 | 04T-I1R72-1-25-96 | Affinity | Integra | Ni Sepharose 6 Fast Flow | Ni-IMAC |
Affinity IMCStips for Integra
IMCStips come with predefined pipetting protocols which can be uploaded to INTEGRA pipettes using VIALINK software which interfaces with the VIAFLO 96, ASSIST and ASSIST PLUS electronic pipette platforms. To ensure the seamless integration of IMCStips, IMCS provides technical support and templated scripts along with user guidelines for each application.
Results below were obtained using IMCStips on an ASSIST (P/N: 04T-I1R72XL-1-25-8).
Increased protein recovery comparable to spin columns
Percent recovery of recombinant dasher-GFP using either IMCStips or spin column.
Increased binding across a wide range of concentrations
Kinetic data of various concentrations of GFP (115-700 μg) over 25 binding cycles.
Fraction eluted is independent of volume or cycle number
The impact of number of elution cycles and elution volume on fraction of bound protein eluted.
High specificity across different proteins
Binding dynamics of various His-tagged proteins in IMCStips. SDS-PAGE of purified proteins shown (inset). (A) Recombinant dasher-GFP (GFP). (B) Recombinant arylsulfatase (PaS). (C) Recombinant beta-glucuronidase (GusA). For A, B, C, 1st lane: protein in cell lysate; 2nd lane: flow-through; 3rd lane: eluted sample using IMCStips.
