IMCS

A leader in recombinant enzymes and automated micro-chromatography technologies

Image of blue rendered molecular structure

Antibody Purification

Comparative IMCStip-Based Protein A Purification on the Tecan Fluent: Impact of Resin and Elution pH on Antibody Aggregation

Automated optimization of antibody purification is highly advantageous for
enhancing efficiency and reproducibility in protein purification workflows.

In this application note, Affinity and SizeX IMCStips are used on a Tecan Fluent
system to automate the purification and subsequent buffer exchange of
monoclonal antibodies from clarified cell culture supernatants. The evaluation
specifically examines two protein A resins (MabSelect™ PrismA and Praesto™
Jetted A50 HipH) across varying elution pH levels, focusing on recovery and
aggregation impacts. Results demonstrate the influence of resin choice and
pH conditions on purification outcomes, thus highlighting the value of
automating with IMCStips in streamlining process development workflows.

Read moreComparative IMCStip-Based Protein A Purification on the Tecan Fluent: Impact of Resin and Elution pH on Antibody Aggregation

High-Throughput Purification and Desalting of His-Tagged Proteins on the Analytik Jena CyBio Felix

Generating assay-ready proteins depends on two key steps: isolating the
protein of interest from complex starting material and ensuring the purified protein is in an appropriate buffer. Here we describe a high-throughput workflow for the purification and buffer exchange of his6-tagged proteins. Using Ni-IMAC IMCStips® followed by SizeX150 IMCStips® on the Analytik Jena CyBio FeliX, his6-tagged GFP was successfully isolated from bacterial lysate with >80% recovery and >95% purity from affinity purification and >96% reduction in small molecule contaminants with SizeX buffer exchange. This streamlined approach provides a reproducible and efficient route to prepare proteins suitable for downstream applications, reducing the variability and labor typically associated with manual workflows.

Read moreHigh-Throughput Purification and Desalting of His-Tagged Proteins on the Analytik Jena CyBio Felix

Automated Antibody Purification and Buffer Exchange Using Protein A and SizeX150 IMCStips® on the Opentrons Flex® 

High-quality antibody preparation requires both efficient purification and effective buffer exchange, but conventional workflows are often time-consuming and inconsistent. IMCS has developed validated, automation-ready methods that combine Protein A IMCStips® for selective IgG capture with SizeX150 IMCStips® for rapid buffer exchange and small molecule removal. Implemented on the Opentrons Flex®, these methods deliver >80% recovery and >95% purity in a streamlined workflow that minimizes operator time while supporting flexible throughput. By reducing complexity and ensuring reproducible results, these methods enable laboratories to achieve formulation-ready antibodies faster and more reliably than manual approaches.

Read moreAutomated Antibody Purification and Buffer Exchange Using Protein A and SizeX150 IMCStips® on the Opentrons Flex®