IMCS

A leader in recombinant enzymes and automated micro-chromatography technologies

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Protein A Chromatography

High-Throughput Purification and Desalting of His-Tagged Proteins on the Analytik Jena CyBio Felix

Generating assay-ready proteins depends on two key steps: isolating the
protein of interest from complex starting material and ensuring the purified protein is in an appropriate buffer. Here we describe a high-throughput workflow for the purification and buffer exchange of his6-tagged proteins. Using Ni-IMAC IMCStips® followed by SizeX150 IMCStips® on the Analytik Jena CyBio FeliX, his6-tagged GFP was successfully isolated from bacterial lysate with >80% recovery and >95% purity from affinity purification and >96% reduction in small molecule contaminants with SizeX buffer exchange. This streamlined approach provides a reproducible and efficient route to prepare proteins suitable for downstream applications, reducing the variability and labor typically associated with manual workflows.

Read moreHigh-Throughput Purification and Desalting of His-Tagged Proteins on the Analytik Jena CyBio Felix

Automated Antibody Purification and Buffer Exchange Using Protein A and SizeX150 IMCStips® on the Opentrons Flex® 

High-quality antibody preparation requires both efficient purification and effective buffer exchange, but conventional workflows are often time-consuming and inconsistent. IMCS has developed validated, automation-ready methods that combine Protein A IMCStips® for selective IgG capture with SizeX150 IMCStips® for rapid buffer exchange and small molecule removal. Implemented on the Opentrons Flex®, these methods deliver >80% recovery and >95% purity in a streamlined workflow that minimizes operator time while supporting flexible throughput. By reducing complexity and ensuring reproducible results, these methods enable laboratories to achieve formulation-ready antibodies faster and more reliably than manual approaches.

Read moreAutomated Antibody Purification and Buffer Exchange Using Protein A and SizeX150 IMCStips® on the Opentrons Flex®