In collaboration with
Tymora's Superior Proteomics Services
Discover the full capabilities of your samples with Tymora Analytical's Proteomics Services. Using their proprietary EVtrap technology, Tymora provides a comprehensive analysis of proteins and their post-translational modifications (PTMs) from various biological samples. Identify critical biomarkers and gain a deeper understanding of intricate biological processes.
Whether you're working with plasma, urine, saliva, or tissue samples, Tymora's Proteomics Services provide a reliable platform for your research requirements. Here's what Tymora offers:
Plasma
Probe the comprehensive protein and PTM profile of plasma-derived EVs.
Identify >1,000 unique proteins from just 10 µL plasma, or 600-1,000 unique phosphoproteins from just 0.5 mL plasma.
Urine
Harness the power of truly non-invasive urine samples to uncover unique biomarkers.
10 mL of urine can detect 3,200 phosphopeptides and 1,200 phosphoproteins.
Saliva
Explore the potential of saliva as a rich source of disease-specific EVs.
Identify >1,000 unique proteins from just 20 µL saliva, or 500-1,000 unique phosphoproteins from just 1 mL saliva.
Tissue
Unearth the complex interplay of proteins and their modifications within specific tissues.
Analyze low-abundant proteins, phosphoproteins, glycoproteins, and others from frozen tissue samples.
Cell Lysate
Get an in-depth view of the proteomic landscape of your cell cultures.
Analyze low-abundant proteins, phosphoproteins, glycoproteins, and others from frozen cells or cell lysate samples.
Other Samples
Tymora accommodates a wide range of sample types, catering to your diverse research needs.
Custom services are available for most sample types (e.g., plant, fungi, bacteria, IP, in-gel digests, etc.).
Test EVtrap NOW!
Mention IMev2023 for a special discounted rate!
Discover Powerful, Non-Invasive Biomarkers
Unlock new frontiers in non-invasive biomarker discovery with Tymora Analytical. Extracellular vesicles (EVs), including exosomes, offer a unique window into the health status of their originating cells by their distinct protein profiles. When isolated from urine, they present a wealth of clinically relevant data. Tymora Analytical stands at the forefront of this pioneering approach, using their proprietary EVtrap technology and Proteomics/PTMs analysis. By extracting and profiling proteins and their post-translational modifications (PTMs) from EVs in urine, they provide researchers with comprehensive insights into disease processes, helping to advance personalized medicine.
JUMPSTART YOUR PROJECT
Get your results in two to three weeks! Here's how it works:
- Sample Shipment
- Sample Analysis
- Data Processing
- Results Delivery
Comparison of Extracellular Vesicle (EV) Purification and Isolation Methods
| wdt_ID | Principle/Method | Benefits | Disadvantages | Processing Time | Reported Purity | Reported Yield | References |
|---|---|---|---|---|---|---|---|
| 1 | EVtrap | High yield, purity and reproducibility. Compatible with proteomics analysis, automatable, easy to use with standard equipment. |
Higher cost, elution buffer needs to be dried or neutralized. |
< 1 hour | High (>99%) | High (>95%) | |
| 2 | Ultracentrifugation | Current benchmark | Time consuming, low yield, cumbersome process, may damage exosomes |
> 4 hours | Medium | Medium | |
| 3 | Density gradient centrifugation | High purity, avoiding exosomal damage |
Labor-intensive, preliminary preparation and cumbersome operation |
＞16 h | High | Low | |
| 4 | Ultrafiltration | Easy, without special equipment and reagents |
Clogging on filtering membrane, loss of exosomes of small particle diameter |
Generally ＜4 h | High | Medium | |
| 5 | Size exclusion chromatography | Simple, economical, maintain the biological function and structure |
Special columns and packing are required, lipoprotein contamination |
0.3 h for qEV | High | High | 7 |
| 6 | Immunoaffinity | High specificity for exosome subtypes isolation |
Expensive, depending on specificity of the antibody |
4–20 h | High | Medium | 8 |
| 7 | Polymer co-precipitation | Simple operation, suitable for large-volume samples |
Potential contaminants (co-purifying protein aggregates or residuary polymers) |
≈0.3–12 h | Low | High |
(Table modified from Chen J, Li P, Zhang T, Xu Z, Huang X, Wang R, Du L. (2022). Review on strategies and technologies for exosome isolation and purification. Front. Bioeng. Biotechnol. doi: 10.3389/fbioe.2021.811971.)
- Iliuk AB. (2022). Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesicles. Methods Mol Biol. doi: 10.1007/978-1-0716-2341-1_11.
- Iliuk A, Wu X, Li L, Sun J, Hadisurya M, Boris RS, Tao WA. (2020). Plasma-Derived Extracellular Vesicle Phosphoproteomics through Chemical Affinity Purification. J Proteome Res. doi: 10.1021/acs.jproteome.0c00151.
- Wu X, Li L, Iliuk A, Tao WA. (2018). Highly Efficient Phosphoproteome Capture and Analysis from Urinary Extracellular Vesicles. J Proteome Res. doi: 10.1021/acs.jproteome.8b00459.
- Lin S, Yu Z, Chen D, Wang Z, Miao J, Li Q, et al. (2020). Progress in Microfluidics-Based Exosome Separation and Detection Technologies for Diagnostic Applications. Small. doi:10.1002/smll.201903916
- Kamerkar S, Lebleu VS, Sugimoto H, Yang S, Ruivo CF, Melo SA, et al. (2017). Exosomes Facilitate Therapeutic Targeting of Oncogenic KRAS in Pancreatic Cancer. Nature. doi:10.1038/nature22341
- Ding L, Yang X, Gao Z, Effah CY, Zhang X,Wu Y, et al. (2021). A Holistic Review of the State-of-the-Art Microfluidics for Exosome Separation: An Overview of the Current Status, Existing Obstacles, and Future Outlook. Small. doi:10.1002/smll.202007174
- Mohammadi M, Zargartalebi H, Salahandish R, Aburashed R, Wey Yong K, and Sanati-Nezhad, A. (2021). Emerging Technologies and Commercial Products in Exosome-Based Cancer Diagnosis and Prognosis. Biosens. Bioelectron. doi:10.1016/j.bios.2021.113176
- Coumans FAW, Brisson AR, Buzas EI, Dignat-George F, Drees EEE, El-Andaloussi S., et al. (2017). Methodological Guidelines to Study Extracellular Vesicles. Circ. Res. doi:10.1161/CIRCRESAHA.117.309417
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Mention IMev2023 for a special discounted rate!
